New Cell-Level Gel Could Treat or Prevent Gum Disease by fighting Inflammation and Changing Composition of Mouth Bacteria: NYU College of Dentistry


A topical gel that blocks the receptor for a metabolic byproduct called succinate treats gum disease by suppressing inflammation and changing the makeup of bacteria in the mouth. Credit: Yuqi Guo

A topical gel that blocks the receptor for a metabolic byproduct called succinate treats gum disease by suppressing inflammation and changing the makeup of bacteria in the mouth, according to a new study led by researchers at NYU College of Dentistry and published in Cell Reports.

The research, conducted in mice and using human cells and plaque samples, lays the groundwork for a non-invasive treatment for gum disease that people could apply to the gums at home to prevent or treat gum disease.

Gum disease (also known as periodontitis or periodontal disease) is one of the most prevalent inflammatory diseases, affecting nearly half of adults 30 and older. It is marked by three components: inflammation, an imbalance of unhealthy and healthy bacteria in the mouth, and destruction of the bones and structures that support the teeth. Uncontrolled gum disease can lead to painful and bleeding gums, difficulty chewing, and tooth loss. 

“No current treatment for gum disease simultaneously reduces inflammation, limits disruption to the oral microbiome, and prevents bone loss. There is an urgent public health need for more targeted and effective treatments for this common disease,” said Yuqi Guo, an associate research scientist in the Department of Molecular Pathobiology at NYU Dentistry and the study’s co-first author.

Past research has linked increased succinate—a molecule produced during metabolism—to gum disease, with higher succinate levels associated with higher levels of inflammation. Guo and her colleagues at NYU Dentistry also discovered in 2017 that elevated levels of succinate activate the succinate receptor and stimulate bone loss. These findings made the succinate receptor an appealing target for countering inflammation and bone loss—and potentially stopping gum disease in its tracks. 

Strengthening the link between succinate and gum disease

The researchers started by examining dental plaque samples from humans and blood samples from mice. Using metabolomic analyses, they found higher succinate levels in people and mice with gum disease compared to those with healthy gums, confirming what previous studies have found. 

They also saw that the succinate receptor was expressed in human and mouse gums. To test the connection between the succinate receptor and the components of gum disease, they genetically altered mice to inactivate, or “knock out,” the succinate receptor. 

In “knockout” mice with gum disease, the researchers measured lower levels of inflammation in both the gum tissue and blood, as well as less bone loss. They also found different bacteria in their mouths: mice with gum disease had a greater imbalance of bacteria than did “knockout” mice.

This held true when the researchers administered extra succinate to both types of mice, which worsened gum disease in normal mice; however, “knockout” mice were protected against inflammation, increases in unhealthy bacteria, and bone loss.

“Mice without active succinate receptors were more resilient to disease,” said Fangxi Xu, an assistant research scientist in the Department of Molecular Pathobiology at NYU Dentistry and the study’s co-first author. “While we already knew that there was some connection between succinate and gum disease, we now have stronger evidence that elevated succinate and the succinate receptor are major drivers of the disease.”

A novel treatment 

To see if blocking the succinate receptor could ameliorate gum disease, the researchers developed a gel formulation of a small compound that targets the succinate receptor and prevents it from being activated. In laboratory studies of human gum cells, the compound reduced inflammation and processes that lead to bone loss. 

The compound was then applied as a topical gel to the gums of mice with gum disease, which reduced local and systemic inflammation and bone loss in a matter of days. In one test, the researchers applied the gel to the gums of mice with gum disease every other day for four weeks, which cut their bone loss in half compared to mice who did not receive the gel.

Mice treated with the gel also had significant changes to the community of bacteria in their mouths. Notably, bacteria in the Bacteroidetes family—which include pathogens that are known to be dominant in gum disease—were depleted in those treated with the gel.

“We conducted additional tests to see if the compound itself acted as an antibiotic, and found that it does not directly affect the growth of bacteria. This suggests that the gel changes the community of bacteria through regulating inflammation,” said Deepak Saxena, professor of molecular pathobiology at NYU Dentistry and the study’s co-senior author. 

The researchers are continuing to study the gel in animal models to find the appropriate dosage and timing for application, as well as determine any toxicity. Their long-term goal is to develop a gel and oral strip that can be used at home by people with or at risk for gum disease, as well as a stronger, slow-release formulation that dentists can apply to pockets that form in the gums during gum disease. 

“Current treatments for severe gum disease can be invasive and painful. In the case of antibiotics, which may help temporarily, they kill both good and bad bacteria, disrupting the oral microbiome. This new compound that blocks the succinate receptor has clear therapeutic value for treating gum disease using more targeted and convenient processes,” said Xin Li, professor of molecular pathobiology at NYU Dentistry and the study’s lead author. 

Additional study authors include Scott Thomas, Yanli Zhang, Bidisha Paul, Sungpil Chae, Patty Li, Caleb Almeter, and Angela Kamer of NYU Dentistry; Satish Sakilam and Paramjit Arora of NYU Department of Chemistry; and Dana Graves of the University of Pennsylvania School of Dental Medicine.

“Turning Up the Heat” – Heat-resistant nanophotonic material could help turn heat into electricity: U of Michigan


Looking for Renewable Energy Sources “In the Small Things” Contributed By G. Cherry UOM
A new nanophotonic material has broken records for high-temperature stability, potentially ushering in more efficient electricity production and opening a variety of new possibilities in the control and conversion of thermal radiation.
Developed by a University of Michigan-led team of chemical and materials science engineers, the material controls the flow of infrared radiation and is stable at temperatures of 2,000 degrees Fahrenheit in air, a nearly twofold improvement over existing approaches.
The material uses a phenomenon called destructive interference to reflect infrared energy while letting shorter wavelengths pass through. This could potentially reduce heat waste in thermophotovoltaic cells, which convert heat into electricity but can’t use infrared energy, by reflecting infrared waves back into the system. The material could also be useful in optical photovoltaics, thermal imaging, environmental barrier coatings, sensing, camouflage from infrared surveillance devices and other applications.
“It’s similar to the way butterfly wings use wave interference to get their color. Butterfly wings are made up of colorless materials, but those materials are structured and patterned in a way that absorbs some wavelengths of white light but reflects others, producing the appearance of color,” said Andrej Lenert, U-M assistant professor of chemical engineering and co-corresponding author of the study in Nature Photonics (“Nanophotonic control of thermal emission under extreme conditions”).
“This material does something similar with infrared energy. The challenging part has been preventing breakdown of that color-producing structure under high heat.”
The approach is a major departure from the current state of engineered thermal emitters, which typically use foams and ceramics to limit infrared emissions. These materials are stable at high temperature but offer very limited control over which wavelengths they let through. Nanophotonics could offer much more tunable control, but past efforts haven’t been stable at high temperatures, often melting or oxidizing (the process that forms rust on iron). In addition, many nanophotonic materials only maintain their stability in a vacuum.
The new material works toward solving that problem, besting the previous record for heat resistance among air-stable photonic crystals by more than 900 degrees Fahrenheit in open air. In addition, the material is tunable, enabling researchers to tweak it to modify energy for a wide variety of potential applications. The research team predicted that applying this material to existing TPVs will increase efficiency by 10% and believes that much greater efficiency gains will be possible with further optimization.
The team developed the solution by combining chemical engineering and materials science expertise. Lenert’s chemical engineering team began by looking for materials that wouldn’t mix even if they started to melt.
“The goal is to find materials that will maintain nice, crisp layers that reflect light in the way we want, even when things get very hot,” Lenert said. “So we looked for materials with very different crystal structures, because they tend not to want to mix.”
They hypothesized that a combination of rock salt and perovskite, a mineral made of calcium and titanium oxides, fit the bill. Collaborators at U-M and the University of Virginia ran supercomputer simulations to confirm that the combination was a good bet.
John Heron, co-corresponding author of the study and an assistant professor of materials science and engineering at U-M, and Matthew Webb, a doctoral student in materials science and engineering, then carefully deposited the material using pulsed laser deposition to achieve precise layers with smooth interfaces. To make the material even more durable, they used oxides rather than conventional photonic materials; the oxides can be layered more precisely and are less likely to degrade under high heat.
“In previous work, traditional materials oxidized under high heat, losing their orderly layered structure,” Heron said. “But when you start out with oxides, that degradation has essentially already taken place. That produces increased stability in the final layered structure.”
After testing confirmed that the material worked as designed, Sean McSherry, first author of the study and a doctoral student in materials science and engineering at U-M, used computer modeling to identify hundreds of other combinations of materials that are also likely to work. While commercial implementation of the material tested in the study is likely years away, the core discovery opens up a new line of research into a variety of other nanophotonic materials that could help future researchers develop a range of new materials for a variety of applications.
Source: By Gabe Cherry, University of Michigan

UCLA: ‘Glass bubble’ nanocarrier boosts effects of combination therapy for pancreatic cancer


The nanocarrier’s hollow glass bubble (white, at left) is packed with irinotecan (green) and is covered by lipid layers (blue) that contain the immue-boosing drug 3M-052 (orange particles in close-up image on right). Credit: CNSI/UCLA

Over the past 30 years, progress in early detection and treatment of cancer has helped reduce the overall death rate by more than 30%. Pancreatic cancer, however, has remained difficult to treat. Only 1 in 9 people survive five years after diagnosis, in part because this cancer is protected by biological factors that help it resist treatment.

In hopes of turning the tide, UCLA researchers have developed a technology that delivers a combination therapy to pancreatic tumors using nanoscale particles loaded with irinotecan, a chemotherapy drug approved as part of a drug regimen for pancreatic cancer, and 3M-052, an investigational drug that can boost immune activity and help overcome tumors’ resistance.

In a study recently published in the journal ACS Nano, the research team showed that the simultaneously delivered combination outperformed the sum of its parts in a mouse model of pancreatic cancer.

“In my opinion, invoking the immune system will make a big difference in providing a much better treatment outcome for pancreatic cancer,” said corresponding author André Nel, a distinguished professor of medicine and director of research at the California NanoSystems Institute at UCLA. “That’s where I hope this research is taking us.”

The researchers’ double-loaded nanocarrier was more effective at shrinking tumors and preventing cancer metastasis in mice than either irinotecan without a nanocarrier or nanocarriers that delivered the two drugs independently. The combination therapy also attracted more cancer-killing immune cells to tumor sites and maintained drug levels in the blood for longer. There was no evidence of harmful side effects.

In addition to blocking cancer cells from growing, irinotecan sends a danger signal to the immune system‘s dendritic cells; these in turn mobilize killer T cells, which travel to tumor sites and destroy cancer cells. But because dendritic cells are often functionally impaired in patients with pancreatic cancer, 3M-052 provides extra assistance, helping them better marshal killer T cells both at the cancer site and in nearby lymph nodes.

Combination therapies for cancer are not new, but packaging drugs together in the same nanocarrier has proven difficult. Only one dual-delivery nanocarrier for chemotherapy has been approved by the Food and Drug Administration. However, over the past seven years, the Nel lab has developed an approach for simultaneous delivery, and the current findings provide further evidence that their innovative nanocarrier design enables the drugs to work in tandem more effectively than if they were delivered separately.

Most nanocarriers are composed of layers of lipid molecules made up of fatty substances, similar to a cell membrane, with spaces into which drugs can be packaged. With the new device, that double layer of lipids surrounds a core glass bubble made of silica whose hollow interior can be filled with irinotecan. In an ingenious twist, UCLA postdoctoral researcher and first author Lijia Luo figured out that the 3M-052 molecule’s fatty tail could be used for integrating the second drug directly into these outer lipid layers.

The structural design of the carrier, which is so small that it would take 1,000 of them to span the width of a human hair, helps prevent drug leakage and toxicity while the device enters a formidable ropelike barrier protecting the pancreatic cancer and travels to the tumor site. The glass bubbles offer additional protection from leakage, enabling the carrier to deliver more irinotecan to the tumor site, compared to other drug carriers.

The team will conduct further preclinical experiments to test their treatment in large-animal models and confirm quality-control for large-scale manufacturing of their silica nanocarriers.

“It traditionally takes 10 to 20 years for new breakthrough technologies to reach the marketplace,” said Nel, who is also founder and chief of UCLA’s nanomedicine division and director of the University of California’s Center for Environmental Implications of Nanotechnology. “Nanocarriers have been around for almost 20 years. While lipid-based nanocarriers are leading the way, the silica-based carrier decorated with lipid layers stands a good chance of speeding up the rate of discovery and improving cancer immunotherapy.”

BATTERY RECYCLING: THE OTHER BIG INDUSTRY ON EUROPEAN HORIZON


The growth of recycling plants in Europe is a necessary environmental response to the increasing demand for batteries for electric vehicles and the gigafactory industry that will develop in the coming years.

*** Contributed by: M. Guitierrez of CICenergiGUNE

The entire world is currently immersed in an energy transition that involves, among other things, a complete electrification of the mobility sector and the promotion of renewable energies. As a result, the demand for batteries has grown steadily by 30% annually in recent years and the outlook for the coming years is exponential.

The main driver of this growth is the electric vehicle, which is expected to represent more than 88% of the demand compared to other types of applications. Moreover, it is estimated that two out of three vehicles will be electric by 2040. Hence, Europe, which seeks to be a benchmark in this new scenario, is taking positions through the creation of more and more gigafactories.

However, this increase in the manufacture and use of batteries for electric cars requires the development of a new and increasingly necessary sector: the recycling of these batteries. Above all, taking into account that the energy transition to be faced in the coming years is linked to the circular economy which is essential for the desired change towards sustainability.

According to a Greenpeace study, almost 13 million tons of batteries from electric vehicles will reach the end of their life between 2021 and 2030. This represents a huge environmental impact due to the amount of critical materials (lithium, cobalt, nickel…) that will have to be disposed of. And even more so, taking into account that the manufacture of new batteries will require the extraction of around 10 million tons of new materials.

The current situation in Europe in terms of material recycling is still far from what is desirable, given that today only 22% of cobalt, 16% of nickel, 12% of aluminum and 8% of manganese are recycled.

That is why, as we have seen in previous blog articles, great efforts are being made to study how these materials can be reused and/or recycled, in order to promote a circular economy.

Source: ReCell Center

Europe seeks to regulate this macro-industry through a new regulatory framework

One of the major efforts made in recent months has been focused, in a forward-looking approach, on the development of regulations to control the end of the life of these batteries.

Europe has already taken action on the matter through a proposal to change the current regulatory framework, not only to develop the “circularity” of the market, but also to reduce dependence on third territories as far as the supply of raw materials is concerned.

It is a proposal that includes thirteen major blocks of measures covering the entire value chain of the industry with special emphasis on the efficiency levels of recycling and recovery of materials. The objective is to contribute to the protection, preservation and improvement of the quality of the environment by minimizing the negative impact of batteries and capacitors and their waste.

To achieve these goals, the European Directive prohibits the placing of batteries containing certain hazardous substances on the market and defines measures to establish systems aimed at achieving a high level of collection and recycling. It also aims to improve the environmental performance of all operators involved in the life cycle of batteries, such as producers, distributors and end users and, in particular, operators directly participating in the treatment and recycling of waste batteries and capacitors.

The U.S. regulation, on the other hand, complains about the absence of a standardized procedure for the design, materials and chemistries of the batteries that are manufactured. Their proposal includes the introduction of a standardized procedure for battery recycling to help manufacturers understand which materials and designs are most easily recyclable. This is known as the “Designed for Recycling” concept.

In this regard, Spain has the Royal Decree 20/2017, of January 20, which obliges manufacturers to inform consumers about the criteria that will be adopted to ensure that the vehicle they are purchasing will be treated responsibly at the end of its useful life.

Leading international players join the recycling wave

The battery recycling sector requires a transformation and there are many European players that are betting on it to boost the circular economy and create a competitive advantage associated with the knowledge of this growing industry.

One of them is ERMA (European Raw Materials Alliance); an alliance that includes companies, associations, universities and research centers –among them CIC energiGUNE– focused on the recycling industry, and whose activities include, among others, supporting the capacity of the European raw materials industry to extract, design, manufacture and recycle materials.

Among the agents belonging to ERMA, we find the RECHARGEassociation, which mainly brings together large companies and some associations related to the materials used in batteries, with the intention of promoting and defending the interests of the entire value chain.

Another player, this time directly linked to battery recycling, is Reneos. This is the first European platform for the collection and recycling of electric vehicle batteries. This platform focuses its activity on the collection of batteries and waste in compliance with European guidelines, before giving them a second life through reuse or disassembly for recycling.

Finally, it is worth mentioning other alliances or initiatives that defend to a greater or lesser extent the interests of the recycling industry. Some of them are Eucobat, the European association of national battery collection systems; EBRA, a grouping that aims to develop the highest levels of professionalism in the battery recycling industry; and EuRIC, which, thanks to its strong network of European and national recycling associations, acts as a trusted interface between the industry and the European Union for the exchange of best practices in all matters related to recycling.

Europe´s proliferation of recycling plants

Given the need, sustainability and also the profitability of the battery recycling industry, more and more companies are commercializing new processes for the collection, discharge and dismantling of these batteries.

Not surprisingly, according to a study by the consulting firm Yole Development, during the period from 2020 to 2025 a CAGR of 25% is estimated in the global value of the recycled materials industry for lithium-ion batteries. This would mean, in economic terms, a total market value of close to $1.2 billion by 2025, and some even forecast that, by 2040, this market will reach a value of almost $24 billion.

In Europe, this spread of battery recycling projects is spearheaded by the factory that SMS Group wants to set up together with the Australian company Neometals. It is called “Primobius” and promises effective recycling of lithium-ion batteries.

Meanwhile, Solvay and Veolia are continuing to advance their battery recycling partnership, which began in September 2020, and have announced the establishment of a demonstration plant for recycling battery materials.

In Northern Europe, Sweden has announced the project of a new battery recycling plant, with an investment of more than €24 million by Stena Recycling and will be located in the town of Halmstad.

At the same time, in Central Europe, Volkswagen has recently opened a pilot plant in Salzgitter (Germany) and also, the recycling company Elemental Holding has announced an investment of 182 million euros for the treatment of batteries and other metals containing waste in Poland.

If we focus on southern Europe, recently, the companies Endesa and Urbaser have announced that Spain will have its own battery recycling plant in León in 2023. A project that promises the treatment of 8,000 tons of batteries per year that will be processed through a separation and shredding procedure that will allow the recycling of the materials of the storage system.

In addition to those already mentioned, other plans have been announced for the creation of recycling plants. One of them is Northvolt, which intends to start up a factory capable of recycling 25,000 tons of batteries per year, and also, the one of BASF in Germany, both with the intention of being operational next year.

The alternative to recycling: the second life of batteries

Another trend that has arisen as a result of the increased use of batteries is the possibility of reconditioning electric vehicle batteries as an energy storage solution for other applications. This is known as “Second Life Batteries“.

Indeed, if the useful life of an electric vehicle battery is estimated at around 8 years, the energy remaining inside the battery cells can be extended by 5 to 10 years, depending on the application in which it is used, until it finally reaches its end of life.

This has led to initiatives such as the one of Enel Group, which has used 90 used Nissan Leaf batteries in an energy storage facility in Melilla. Meanwhile, the energy company Powervault has announced its partnership with Renault to equip domestic energy storage systemsbased on batteries from retired electric vehicles.

Not only that, Spain has also been a pioneer in Europe by installing the first chargers powered by second-life batteries on the highway linking Madrid and Valencia.

One way or another, the premise is clear. It is necessary to find a solution for the recycling of around 50,000 tons of batteries that are expected to be discarded from 2027; a figure that could even multiply and reach 700,000 tons in 2035.

Hence, one of the main focuses of work and research at centers such as CIC energiGUNE is the advancement of techniques and solutions that promote the development of the recycling industry. Even more so if we want to ensure that the battery sector becomes a reference in terms of sustainability.

Below, as a summary, from CIC energiGUNE we have gathered the classification of the main agents that have announced to be associated to battery recycling:

Read Genesis Nanotech Online: A Deep Dive into the Development of a textile based protein sensor for monitoring the healing progress of wounds


This article focuses on the design and fabrication of flexible textile-based protein sensors to be embedded in wound dressings.

Chronic wounds require continuous monitoring to prevent further complications and to determine the best course of treatment in the case of infection. As proteins are essential for the progression of wound healing, they can be used as an indicator of wound status. Through measuring protein concentrations, the sensor can assess and monitor the wound condition continuously as a function of time.

The protein sensor consists of electrodes that are directly screen printed using both silver and carbon composite inks on polyester nonwoven fabric which was deliberately selected as this is one of the common backing fabric types currently used in wound dressings. These sensors were experimentally evaluated and compared to each other by using albumin protein solution of pH 7. A comprehensive set of cyclic voltammetry measurements was used to determine the optimal sensor design the measurement of protein in solution. As a result, the best sensor design is comprised of silver conductive tracks but a carbon layer as the working and counter electrodes at the interface zone. This design prevents the formation of silver dioxide and protects the sensor from rapid decay, which allows for the recording of consecutive measurements using the same sensor.

The chosen printed protein sensor was able to detect bovine serum albumin at concentrations ranging from 30 to 0.3 mg/mL with a sensitivity of 0.0026𝜇0.0026μA/M. Further testing was performed to assess the sensor’s ability to identify BSA from other interferential substances usually present in wound fluids and the results show that it can be distinguishable.

Introduction

Skin is a crucial organ of the human body as it acts as a barrier to protect the rest of the body’s tissues and organs1, therefore when it suffers an injury, other essential and healthy organs could become infected or injured2,3. While in most minor wound cases, minimal intervention is required such as placing a bandage or medical gauze to prevent further damage to the wound and to prevent it being overrun by infectious microorganisms. However, many chronic wounds need to be monitored and retreated constantly over long periods of time.

The cost of treating wounds is a critical issue as it is estimated to account for at least 3%3% of the total healthcare expenditure in most developed countries4. Since 2018, it is estimated that the UK is managing approximately 3.8 million patients with a wound in a clinical setting annually5. It was estimated that health services in 2012 spent ££5.1 billion on costs associated with wound care management6 which provided a compelling case for improvement in the current standard of wound dressings not only to reduce healthcare costs but also to improve patient quality of life7. However, better and effective means of reporting quantitative information about the wound condition in real time is required to inform and guide treatment decisions as improved wound care will deliver improved public health and healthcare costs8,9.

The wound healing process can be monitored by repeatedly determining the multiple physiological changes that occur including but not limited to pH, alkalization, temperature, uric acid and specific protein types such as albumin and fibrinogen whilst tissue repair progresses2. Detection of these biomarkers with minimally invasive techniques can provide an effective way for the real-time monitoring of the condition of a wound. In addition, remote wound monitoring could keep the patient informed about their condition, improve their quality of life and reduce the frequency of face-to-face consultations and treatments with healthcare providers10.

Higher precision in wound detection treatment is advancing more rapidly as presented recently3where an integrated wound recognition strategy is conducted by extracting patterns of specific irregular wounds. This work was implemented on a bandage, but others have investigated different textiles to allow flexibility of wearable medical devices11,12,13. Integrating electronics with textiles has advanced medical care by facilitating multiple physiological parameters and the body’s biomolecular state to be monitored remotely through minimal or noninvasive techniques and sometimes with reduced direct contact with the human body13,14. Improved flexibility and durability allow electronic devices to be more suitable for wearable biosensors as they can be embedded into clothing to realize electronic textiles15. These emerging technologies in wearable electronics have made using smart textiles in the design of flexible patches using textiles11 which made wound dressings more achievable and is leading to major advances in healthcare monitoring, personalized therapy, and human-machine interaction12. The aim of wearable textile biosensors is to indirectly detect critical physiological changes in the body through measuring indirect stimuli that can be readily detected from outside the body despite the uncontrolled environment surrounding the body16. However, to be embedded in wound dressings, not only does it have to be miniaturized but it also needs to be flexible and lightweight to make positioning around the wound feasible and as comfortable to wear as possible regardless of the location of the wound17. To provide better flexibility the ink used in fabricating the biosensor plays a critical role as adding a bendable layer to a textile can provide fabric reinforcement and make it mechanically bendable18.This is one of the challenges that were addressed in this paper and will be discussed in the later section.

The design of the protein sensor is based on the structure of an electrochemical cell that uses a three-electrode configuration to perform the cyclic voltammetry measurements19,20,21.

Electrochemical sensors are generally preferred because they can provide rapid real time monitoring of change and wound conditions, they are also relatively inexpensive and can be miniaturized and embedded within textiles22. Categorically the device should be a potentiometric biosensor which works by measuring the voltage produced when electric current flows through the solution under static conditions23,24,25,26,27,28.

Screen printing technology has been used to fabricate electrochemical electrodes on ceramic or plastic based substrates29,30,31.This technique involves forcing suitable ink formulations in paste form through a patterned stencil or screened mesh of a specific size and shaped using a squeegee to form the desired design on a substrate32. Screen printing provides greater design freedom in that the printed layers can have any orientation on the fabric and do not need to follow the yarn directions. In addition, screen printing provides the ability to produce arrays of the same or different devices in a straightforward fashion; screen printing is inherently a batch process producing multiple devices from a single screen design.

While pH and temperature have been used as parameters in assessing wound status33,34,35, detecting protein concentrations helps to identify wound healing stages36 as it is less likely to be affected by the active external environment surrounding the exudate. Albumin was the protein determined in this research work, which has been modelled and measured previously37,38 as it is the most abundant protein in blood plasma (it represents 50%50% of total protein)39, previous research has shown a relation between the wellness of a person and the albumin concentration40, establishing albumin concentration as a good marker of protein concentrations in wounds. Albumin concentrations in wounds have been used as indicator of wound severity conditions, for which a concentration of >15>15 mg/mL is in inflamed wounds41. While the protein level in a healing wound is around 9 mg/mL, compared to levels of 35 mg/mL for chronic slow healing wounds42.

Bovine serum albumin (BSA) was used to prepare standard solutions of albumin43. The use of BSA as a protein source specifically when testing electro-chemical sensors has been previously reported by others44 because the concentration can be easily standardized and altered precisely to evaluate the detection range. Most importantly, the properties of BSA are very similar to human serum albumin with respect to other proteins as discussed in a recent paper38.

For the first time in literature, this research presents a unique approach to integrate protein sensors in fabric which improves the sensors durability, comfort, flexibility and wearability and performance within specification. Although a similar approach was presented recently8,45, where screen printed electrodes (SPEs) were printed on a paper and placed inside a bandage but measure pH and uric acid to monitor chronic wounds.

In this research, three designs were investigated to determine the optimal biosensor design for integration into wound dressings. These designs were fabricated by screen printing. Each design was printed directly on 3 different types of fabric with different surface roughness and layer thickness. The conductive tracks were made from silver flake-based ink and carbon ink. UV curable dielectric ink was used to smooth out the textile surface and to provide an even surface for further printing. The same UV ink was used as the encapsulation. 2-point resistance measurement was conducted on printed layers to ensure continuity and cyclic voltammetry measurements were performed to determine sensitivity and selectivity.

Results and discussion

The research had two main stages. The first stage encompassed the design and fabrication of the textile based sensors and the second stage covered the testing of the sensors using a previously established empirical technique reported by the authors37.

Fabrication of textile based screen printed electrodes

The process of screen printing on fabric involves four stages as illustrated in Fig. 1 which shows an exploded view of the three designs. The first design purely consisted of silver layer as both the conductive tracks and electrodes; the second consisted of a mix of both silver and carbon layers; the third design had a carbon layer as part of the electrodes in the region where the sensor encounters the fluid under test at the interface zone. In all three designs, there were three electrodes (working, counter, reference electrodes). The dimensions of the sensors are shown in Fig. 2.

The sensors were printed onto three textiles, two of which are medical fabric types. The first one (Type A) was a polypropylene non-woven fabric and has areal surface roughness (Sa =  119.24 119.24 µm), the second (Type B) is a blend of cotton/polyester woven fabric and has areal surface roughness (Sa =  59.37 59.37 µm) while the third one (Type C) is made from polyester non-woven fabric and has areal surface roughness (Sa =  151.52 151.52µm). Each fabric was placed and adhered in turn onto an alumina tile which provided a rigid platform for printing. The sensors were successfully printed on all three fabric types but it is discovered that printing on the cotton/polyester fabric was easier, while Type A fabric was more difficult to print on because it started to tear apart. However, using polyester non-woven fabric (Type C), which is similar to the one used in wound dressings, prevented the initial substrate layer from cracking. Upon fabrication, the final printed sensors are shown in Fig. 3. After printing, the resistance between two end points of printed track was measured using a multimeter. The resistance observed in design A and B (shown in Fig. 1) was less than 1 ohm on average, while design C always had a much higher resistance (in the range of 260–410 ohms). Microscopic images of the three designs are shown in Fig. 1 in that the tracks were well defined.

figure 1
Figure 1
figure 2
Figure 2
figure 3
Figure 3

The main challenge in the printing process was to maintain the correct alignment when printing each layer to prevent short circuit and to preserve the sensor design. To resolve this issue, a trial print was deposited on a transparent laminate sheet before every deposit to make sure the patterns were all perfectly aligned before printing directly on fabric. The second main challenge faced repeatedly was the roughness of each printed layer. To print several layers on top of each other, all layers need to be smooth with no pin holes on the surface as this affected the roughness of the final finishing layer. To address this issue, the smooth side of the fabric was initially shaved to enable the surface to be as uniform as possible. The dielectric layer was then printed several times with varying printing gaps to reduce any pin holes and to provide an even platform for printing conductive layers. The third challenge was that the fabric could not be easily removed from the alumina (supporting platform). It was observed that this only occurred when using thin fabric such as Type A fabric shown in Fig. 3. To avoid this issue, two layers of the same fabric were adhered and fixed on top of each other. This strengthened the fabric layer but also eased the removal of fabric after printing.

The printed layers after each stage are shown in Fig. 4. Initially, in the first stage shown in (A), the polymer interface layer was deposited six times to create a smooth platform for subsequent layers to be printed on top. The interface layer was then cured under UV light to produce a thickness of  110 110 µm. Next, the silver conductive electrodes were printed as shown in (B) and cured in the oven for 15 minutes at 100 ∘∘C. The silver layer was printed twice to produce a thickness of  16 16 µm. The carbon layer was then printed only on the second and third designs as shown in Fig. 4C and cured in the oven for 15 minutes at 100 ∘∘C. The print was repeated twice to create a thickness of  26 26 µm. Finally, the same material used for the interface layer was printed to protect the conductive tracks of the sensors as shown in (D) and was cured under UV light to produce a thickness of  14 14 µm. A microscopic image of the interface for each design before being tested is shown in Fig. 5. The images captured show how the electrodes in delicate area of the interface zone are clearly separated from each other and how the layers are well aligned and printed on top of each other and there is no short circuit occurring.

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Figure 4
figure 5
Figure 5

Upon fabrication, the sensors were highly flexible and can be easily bent as shown in Fig. 6, where the sensor was wrapped around cylinders with a diameter ranging from 1 mm to 10 mm to mimic the bending when worn on patient. The results showed that all designs could wrap around these diameters without causing cracks in any of printed layers. This was further cross validated through continuity measurement, proving the conductivity of printed sensors is intact. This feature is of great significance because of the nature of the targeted application as the exact positioning of a wound is unpredictable, and the dressing does need to be wrapped and bent around the human body. To test the bendability of the sensor, an experimental setup was prepared as shown in Fig. 7. This was achieved using a Shimadzu AG-X Universal Testing Machine with custom 3D printed attachments to grip the printed sensors. The two attachments were initially set at 21mm apart which is the maximum distance from two opposite sides of the sensor.

These attachments were them moved towards each other and stopped at mm apart to avoid damage to machine and attachment. The cyclic test was repeated 5 times with 3 sensors. The measured force to bend the sensor ranges from 0 to 2.5 N depending on compression position and there was no damage to the integrity of all designs which was subsequently cross-validated through continuity measurement. This mechanical test demonstrated a quantifiable measure of the sensor ability to bend with minimal force without affecting the sensor’s structure. After the test was conducted, the conductivity of the electrodes was measured and remained unchanged and unaffected by the test.

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Figure 6
figure 7
Figure 7

Cyclic voltammetry (CV) measurements

The fabric around the bottom edge of the sensor was removed in order to be connected to the AUTOLAB Dropsens adaptor which includes an insertion connector with 3 pins. BSA with 8 concentrations ranging from 0.3 – 30 mg/mL were used as the protein sources. Initially, the experiment was conducted on three sensor designs on all three types of fabric to establish the most suitable design. For each measurement, the current at the oxidation peak was observed and recorded and a best fit line of all the measurements was then plotted in Fig. 8. The SPE design with only silver layer at the interface zone (SPE design (A)) stopped functioning when testing a solution with concentration below 3 mg/mL. As shown in Fig. 8, the two cycles conducted using SPE with only carbon were close to each other in terms of gradient as there was less than 8%8% difference in comparison with design A and B where the differences in measurements between two cycles were 32%32% and 41%41%, respectively. This demonstrated that design C was the most stable and reliable design as it provided reproducible and comparable results each time. The results show a clear relationship between BSA concentration and current peak obtained at the working electrode. This happens because the increase in concentration gradient near the surface of the working electrode causes an increase in the current observed at the oxidation peak. This is a result of the increase in the amount of electroactive proteins adsorption at the interface when the concentration of BSA increases, leading to increase in surface charge density which drives higher current at the oxidation peak46.

figure 8
Figure 8

The status of the sensors after CV measurement performed was investigated to ensure no printed material was damaged. This was achieved by examining each sensor post measurement under the microscope. During the measurement process, the formation of a dark grey color coating (Silver dioxide) was observed at the working electrode when using SPE design (A) as shown in Fig. 9A. This indicated that the silver electrode was damaged before the end of the cycle and could lead to unreliable results. In order to examine the underlying silver layer in design B, the carbon layer was manually removed to expose the silver layer. SPE design (B) where the carbon layer was scratched in SPE design (B) to make the silver layer more visible as shown in Fig. 9B. The image illustrates how silver layer was still oxidized, but was far less visibly damaged than design (A) because it is protected by the carbon layer as it is noncorrosive. The SPE design (C) remained unchanged as there was no silver present at the interface zone. SPE design (C) was then observed using a scanning electron microscope (SEM) and a cross-sectional micrograph including the conductive tracks, the encapsulation layer, interface layer and the textile is shown in Fig. 10. The image shown demonstrates the continuity and consistency of the multiple layers present in design of the sensor around the interface zone.

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Figure 9
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Figure 10

Further empirical testing was only conducted using SPE design (C). The CV measurement obtained after the redox reaction is shown in Fig. 11 when 30 mg/mL BSA was used. After repeating each test three time with new sensor on each fabric type, there was a correlation observed after the first cycle between cycle 2 and 3. The equation of the line for the sensors with three different fabric types are included in Table 1.Table 1 The relationship between number of cycles and CV measurements.

Full size table

The CV experiment was then repeated three times on the same sensor and performed on all three types of fabric. The results obtained using the three types of fabric overlapped each other and were within the same current range at the working electrode (0.06–0.15A). Therefore, the effect of fabric type on the redox reaction was minimal. Type (C) fabric was more repeatable as clearly shown in the graph (the undashed lines) and was chosen as the standard in the fabrication of the sensor because it is also commonly used for medical wound dressings. In addition, the first CV cycle was always lower in magnitude than the measurements obtained in the subsequent cycles. The second and third cycles were consistently closer to each other in comparison with the initial cycle. This is more visible when all peaks of several concentrations of each cycle were compared to each other as shown in Fig. 12. Therefore, as a standard it is best to take the average of the second and third cycles when comparing the results.

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Figure 11
figure 12
Figure 12

Since the combination of type (C) fabric and SPE design (C) was ideal, eight BSA concentrations were used to examine the sensitivity of this combination. The current observed at the oxidation peaks were recorded, shown in Fig. 12. The best fit line was drawn based on the average value around each solution of each cycle. While the initial cycle was consistently lower in gradient as presented in Table 2 than the second and third cycles conducted on the same un-replaced sensor which illustrates that SPE design (C) is the most reproducible.

After testing the sensitivity of the chosen SPE sensor, it was important to analyse the selectivity of printed sensor in comparison with other substances present in wounds. Therefore, the SPE was tested against several potentially interfering substances such as creatin, hydrogen peroxide, glucose and absorbic acid and the results are shown in Fig. 13 similar to a study achieved previously47. In Fig. 13 the shape and range of the redox reaction obtained through CV of BSA is different from the other tested solutions tested which eliminates the effect of interference.

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Figure 13

Scientific Reports volume 12, Article number: 7972 (2022) 

Conclusion

The sensor was fabricated using the screen printing technique and consisted of four layers: an interface layer, silver electrodes, carbon conducting interface area and an encapsulation layer. The sensor relied on quantifying the protein concentration by measuring changes to conducting cyclic voltammetry measurements. After testing three sensor designs with different silver and carbon combinations, it was concluded that that the design with only carbon material presented at the interface zone was the optimal design as not only it provided the most reproducible and consistent results upon testing, but also it remained unaffected by oxidation. The sensor was printed on three different fabric types, and all presented promising results, one of which was wound dressing fabric which was chosen as the substrate as it is commonly used medically. During the screen printing process, precise alignment is a key aspect in the fabrication process to prevent any short circuit of the electrodes and to provide efficient performance of the sensors. Upon further cyclic voltammetry empirical testing and result analysis conducted using carbon only sensors (design (C)) and on Type C fabric, it was determined that to compare the outcome of different BSA concentrations it is best to take the average of the second and third cycles conducted on the same sensor.

The CV measurements demonstrated that the screen printed protein sensor could accurately monitor BSA concentrations from 0.3 to 30 mg/mL with a sensitivity of 0.0026𝜇0.0026μA/M. Additionally, an SEM image was captured and presented in the paper to demonstrate the consistency and continuity of the different layers making up the texture of the final fabricated design. The final design also demonstrated its ability to bend easily around different diameters without breaking. Further testing was conducted to assess the sensor’s ability to detect BSA from other interferential substances present in a wound. The measured results show that the chosen SPE type (C) can successfully distinguishes BSA in the range of 3 to 30 mg/mL from others.

Methods

Screen printing process

Layer types

To design the protein sensors shown in Fig. 6, the sensor consisted of four printed layers. Initially, a layer was built to create an interface for the electrode conductive tracks. Silver layer was then deposited over the interface layer. In two of the designs (design B and C), carbon conductive ink was deposited over the interface zone. Finally, an encapsulation layer was deposited on top of the sensor tracks and around the interface zone to protect the sensor electrodes and to prevent any short circuit. In this work, three different designs were considered when designing the carbon conductive layer.

Ink types

Three types of inks were used: a UV curable polymer ink from ElectraPolymers Ltd was used to act as the interface and encapsulation, carbon and silver inks from Henkel were used as the electrodes and conductive tracks, respectively.

Textile materials

Three different fabric types were tested: 1) a non-woven polypropylene fabric (Type A), 2) a woven polyester/cotton fabric (65%65%/35%35%) (Type B), 3) a non-woven polyester fabric (Type C). Type A and C are commonly used in used in wound dressings and therefore are more favorable. Although the woven polyester/cotton (Type B) fabric is the most ideal type of fabric for printing because it is relatively smoother than the others (has areal surface roughness (Sa) =  59.37 59.37 µm), yet it is not commonly used in wound dressings. We only use Type C as a comparison.

Protein solution preparation

The protein used to test the printed sensors was BSA powder as it is considered as a standard for protein quantification (Sigma-Aldrich, Steinheim, Germany). In this research, 8 samples of BSA solutions were prepared with different concentrations and diluted using deionized water: 30, 23, 18, 11, 7, 3, 1, 0.3 mg/mL with a pH value of 7. Initially, 30 mg/mL was prepared by putting 3 grams of BSA powder in 100 mL of deionized water, and then the rest of the solutions were prepared by diluting the original stock solution.

Measurement setup

A setup similar to the methodology discussed in the previous work by the authors was implemented. The objective is to evaluate the sensitivity shown in Table 2 of the developed textile-based screen printed carbon electrodes as shown in Fig. 14. The printed protein sensor was connected the Metrohm Dropsens device. A glass filled with ice was placed within a close proximity to the sensors to provide humid environment and to prevent evaporation of the BSA samples. This was achieved by conducting a series of cyclic voltammetry experiments on different protein solutions using an AUTOLAB potentiostat device (PGSTAT101). The setup parameters are listed in Table 3.

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Figure 14

Table 3 Parameters of cyclic voltammetry measurement setup.

Full size table

Table 2 The best fit line of oxidation peak measurements with all three designs using type (C) fabric.

Read the Latest Genesis Nanotech Online: Therapeutic viruses help turbocharge the immune system against cancer


The illustration shows a cancer cell (center) surrounded by immune T-cells augmented with an oncolytic (cancer-fighting) virus. A new study describes how a combination of immunotherapy and virotherapy, using myxoma virus, provides new hope for patients with treatment resistant cancers. Credit: Jason Drees

The immune system has evolved to safeguard the body from a wildly diverse range of potential threats. Among these are bacterial diseases, including plague, cholera, diphtheria and Lyme disease, and viral contagions such as influenza, Ebola virus and SARS CoV-2.

Despite the impressive power of the immune system’s complex defense network, one type of threat is especially challenging to combat. This arises when the body’s own native cells turn rogue, leading to the phenomenon of cancer. Although the immune system often engages to try to rid the body of malignant cells, its efforts are frequently thwarted as the disease progresses unchecked.The illustration shows a cancer cell (center) surrounded by immune T-cells augmented with an oncolytic (cancer-fighting) virus. A new study describes how a combination of immunotherapy and virotherapy, using myxoma virus, provides new hope for patients with treatment resistant cancers. 

In new research appearing in the journal Cancer Cell, corresponding authors Grant McFadden, Masmudur Rahman and their colleagues propose a new line of attack that shows promise for treatment-resistant cancers.

The approach involves a combination of two methods that have each shown considerable success against some cancers. The study describes how oncolytic virotherapy, a technique using cancer-fighting viruses, can act in concert with existing immunotherapy techniques, boosting the immune capacity to effectively target and destroy cancer cells.

Oncolytic viruses represent an exciting new avenue of cancer therapy. Such viruses have the remarkable ability to hunt and terminate cancer cells while leaving healthy cells unharmed, as well as enhancing the immune system’s ability to recognize and terminate cancer cells.

One such virus, known as myxoma, is the focus of the current research and an area of expertise for the research group. The study shows that the use of T-cells infected with myxoma virus can induce a form of cancer cell death not previously observed.

Known as autosis, this form of cell destruction may be particularly useful against solid tumors that have proven treatment-resistant to various forms of cancer therapy, including immunotherapy alone.

“This work affirms the enormous potential of combining virotherapy with cell therapy to treat currently intractable cancers,” McFadden says.

McFadden directs the Biodesign Center for Immunotherapy, Vaccines and Virotherapy at Arizona State University.

Internal sentries

The immune system is composed of a range of specialized cells designed to patrol the body and respond to threats. The system is involved in a ceaseless arms race against pathogens, which evolve sophisticated techniques to attempt to outwit immune defenses, propagate in the body and cause disease. Cancer presents a unique challenge to the immune system as tumor cells often lack the identifying cell features that allow the immune system to attack them by distinguishing self from non-self.

Cancer cells can further short-circuit immune efforts to hunt and destroy them, through a range of evasive strategies. Researchers hope to help the immune system to overcome cancer’s notorious tactics of disguise, developing new experimental techniques belonging to a category known as adoptive cell therapy, or ACT.

Such methods often involve removing a collection of cancer-fighting white blood cells known as T-cells, modifying their seek-and-destroy capacities and reinjecting them in patients. Two forms of ACT immunotherapy are described in the new study: CAR T-cell therapy (CART) and T Cell Receptor Engineering (TCR). The basic idea in each case is the same: treating cancer with activated T lymphocytes extracted from the patient.

New method delivers one-two punch to tumor cells

The development of these therapies has been nothing short of revolutionary, and some cancer patients facing grim prospects have made remarkable recoveries following the use of immunotherapy. But techniques like CART and TCR nevertheless have their limitations and are often ineffective against advanced solid tumors. In such cases, cancer cells often manage to evade destruction by T-cells by downregulating or losing the surface antigens or MHC proteins that T-cells use to identify them. 

The new study highlights the ability of immunotherapy when it is coupled with virotherapy to break through the wall of cancer resistance, specifically using myxoma-equipped T-cells. The myxoma can target and kill cancer cells directly but more usefully can induce an unusual form of T-cell directed cell death known as autosis. This form of cell death augments two other forms of programmed cancer cell death induced by T-cells, known as apoptosis and pyroptosis. 

During myxoma-mediated autosis, cancerous cells in the vicinity of those targeted by the therapy are also destroyed in a process known as bystander killing. This effect can considerably enhance the dual therapy’s aggressive eradication of cancer cells, even in notoriously hard-to-treat solid tumors.

A combined myxoma-immunotherapy approach therefore holds the potential to turn so-called “cold tumors,” which fly under the immune system‘s radar, into “hot tumors” that immune cells can identify and destroy, allowing CAR T-cells or TCR cells to enter the tumor environment, proliferate and activate. 

“We are at the edge of discovering newer aspects of the myxoma virus and oncolytic virotherapy,” Rahman says. “In addition, these findings open the door for testing cancer-killing viruses with other cell-based cancer immunotherapies that can be used in cancer patients.” 

The ability to radically reengineer oncolytic viruses like myxoma to target a range of resistant cancers provides a new frontier for the treatment of this devastating disease

+ Explore further

New review highlights cancer-crushing viruses

More information: Ningbo Zheng et al, Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance, Cancer Cell (2022). DOI: 10.1016/j.ccell.2022.08.001

Journal information: Cancer Cell 

Loop Energy Grows European Footprint with UK Expansion


VANCOUVER, BRITISH COLUMBIA and LONDON, UNITED KINGDOM – August 16, 2022 – Loop Energy™ (TSX: LPEN), a designer and manufacturer of hydrogen fuel cells for commercial mobility, will extend its presence in Europe later this month by expanding into the UK.

Loop Energy’s newest facility will be based in Grays, Essex, just east of the centre of London and next to a growing group of manufacturers helping decarbonize road transport, including current customer Tevva Motors, the hydrogen and electric truck OEM which is based in Tilbury.

Loop Energy has already started to recruit for the roles at the new facility, with employees assisting in the areas of production support, customer support and inventory.

The move is in reaction to growing customer demand for Loop Energy’s fuel cells in continental Europe and the UK, where diesel and petrol vehicles will start to be banned from 2030.

Loop Energy, which is listed on the Toronto Stock Exchange, and has raised $100 million CAD so far, is targeting the commercial vehicle sector, including buses and heavy goods vehicles (HGVs).

Diesel and petrol HGVs made up 18% of all road emissions in 2019, amounting to 19.5 metric tons carbon dioxide equivalent (MtCO2e), according to UK government data.

The market for zero-emissions commercial vehicles continues to evolve quickly and Loop Energy is well positioned to provide its technology and expertise to help OEMs and others decarbonize the transportation industry.

The announcement comes just a month after Loop Energy signed a multi-year fuel cell supply agreement with UK-based Tevva, which includes delivery commitments in excess of US$12 million through 2023.

Elsewhere, Loop Energy recently entered the Australian bus market as a supplier of fuel cell modules to Aluminium Revolutionary Chassis Company (ARCC) and the company has seen its order book grow substantially for its technology, with 52 purchase orders in the six months to the end of June, up from 13 over the same period last year.

Loop Energy President & CEO, Ben Nyland said:

“We are excited to open a new facility in the UK, where both the private and public sector is quickly growing around decarbonizing commercial vehicles. We were pleased to see the UK government’s recent commitment to the hydrogen sector, with the Business Secretary’s pledge to unlock £9bn investment needed to make hydrogen a cornerstone of the UK’s greener future,”

“Our investment commitment for the UK market is strategic to serve both UK and the rest of Europe. We expect to service a truck and bus market size upwards of US $15 Billion over the next 2 to 3 years, and our UK facility is established as the localized support center for these vehicles. Our investments to the UK will grow in lock-step with the growth of our local OEM customers, and our investment strategy will align with the timing and volume of our ecosystem partners as the industry ramps up supply to this market,”

“We also believe that the UK’s strong pool of manufacturing and design talent will help take Loop to the next level in its growth story.”

UK Business Minister Lord Callanan said:

“Hydrogen is likely to be fundamental to cutting emissions across some of our largest forms of commercial transport – from buses to heavy goods vehicles. As the world shifts to cleaner transport it is critical we embed a UK supply chain that can capture the economic opportunities of hydrogen technology,”

“Loop Energy’s expansion in Essex is fantastic news for the region, bringing green jobs and growth, while adding to the UK’s reputation as a leader in hydrogen and fuel cell research.”


About Loop Energy Inc.
Loop Energy is a leading designer and manufacturer of fuel cell systems targeted for the electrification of commercial vehicles, including light commercial vehicles, transit buses and medium and heavy-duty trucks. Loop’s products feature the company’s proprietary eFlow™ technology in the fuel cell stack’s bipolar plates. eFlow™ is designed to enable commercial customers to achieve performance maximization and cost minimization. Loop works with OEMs and major vehicle sub-system suppliers to enable the production of hydrogen fuel cell electric vehicles. For more information about how Loop is driving towards a zero-emissions future, visit www.loopenergy.com.
Forward Looking Warning

This press release contains forward-looking information within the meaning of applicable securities legislation, which reflect management’s current expectations and projections regarding future events. Particularly, statements regarding the Company’s expectations of future results, performance, achievements, prospects or opportunities or the markets in which we operate is forward-looking information, including without limitation the ability for Loop to service the truck and bus market and the market’s potential to reach upwards of US $15 billion.

Forward-looking information is based on a number of assumptions (including without limitation assumptions with respect to the potential growth of the bus and truck market and is subject to a number of risks and uncertainties, many of which are beyond the Company’s control and could cause actual results and events to vary materially from those that are disclosed, or implied, by such forward‐looking information. Such risks and uncertainties include, but are not limited to, the market reaching the TAM of upwards of US $15 billion, the realization of electrification of transportation, the elimination of diesel fuel and ongoing government support of such developments, the expected growth in demand for fuel cells for the commercial transportation market and the factors discussed under “Risk Factors” in the Company’s Annual Information Form dated March 23, 2022. Loop disclaims any obligation to update these forward-looking statements.

Source: Loop Energy Inc.

‘Quantum Dot’ Photovoltaic Window Project to receive Funding from U.S. Air force


UbiQD, a nanotechnology company, has revealed that its quantum dot solar technology will be used in a Small Business Innovation Research project with the US Air Force. The contract provides funding for two installations of more than 20 windows and additional scale-up and development funds for the product.

“We are seeing strong fiscal support for sustainability initiatives in the built environment right now,” said CEO Hunter McDaniel. “Our expanded contract with the US Air Force couldn’t come at a better time, right as we are scaling and ahead of the upgraded solar investment tax incentives.”

The company uses luminescent quantum dot tinting to concentrate solar energy and generate electricity while maintaining transparency. Quantum dots are photoluminescent particles so small that it would take 100,000 of them to span one fingernail, said UbiQD. The company said the technology has applications in localized DC microgrids and smart building solutions, including integration with sensors for climate and ambient controls.

Commercial buildings account for 36% of all US electricity consumption at a cost of more than $190 billion annually. Additionally, windows represent 30% of a commercial building’s heating and cooling energy, costing US building owners about $50 billion annually, according to the US Department of Energy.

UbiQD’s quantum-dot tinted window, called WENDOW, has recently been installed in a series of demonstrations projects, including a campus building at the Western Washington University, which the company said is the largest solar window installation to date. The WENDOW can be tinted, allowing for colorful designs. The university installation features vibrant yellow and orange windows. 

“This technology helps Western Washington University get closer to achieving our sustainability goals on campus,” said David Patrick, vice provost for research. “I was impressed by how easily the windows were installed and love how great they look. I’m hoping to see more projects like this on campus in the near future.” 

While the solar windows offer less efficiency than a conventional solar panel, they represent an alternative to blending photovoltaics with the build environment. Read more about solar in uncommon spaces.

UbiQD also builds translucent panels for greenhouses that are integrated with photoluminescent particles that are efficient at converting light into a preferable wavelength. The UbiQD “UbiGro” panels glow a spectrum of color that is optimized for plant growth, absorbing UV and blue light and emitting fruitful orange or red light.

In recent trials, UbiGro led to a 21% boost in flowering in geranium flowers, a 14 to 28% boost in winter strawberry growth, and an 8% yield increase in cannabis production. Increased crop yields are a welcome sign to any grower, and the two companies are set to take that benefit one step further, integrating productive solar PV in the greenhouse-topping modules.

From pv magazine USA **

A New Nanotechnology Application Accelerates the Transition of Stem Cells into Bone – Transforming Regenerative Medicine: Nano Bio-Science from KAUST


A cell cultured on top of the nanowire scaffold. Credit: © 2022 KAUST; Heno Hwang

A nanotechnology platform developed by King Abdullah University of Science & Technology (KAUST) scientists could lead to new treatments for degenerative bone diseases.

The technique relies on iron nanowires that bend in response to magnetic fields. Bone-forming stem cells grown on a mesh of these tiny wires get a kind of physical workout on the moving substrate. They subsequently grow into adult bone considerably quicker than in conventional culturing settings, with a differentiation protocol that lasts only a few days rather than a few weeks.

“This is a remarkable finding,” says Jasmeen Merzaban, associate Professor of bioscience. “We can achieve efficient bone cell formation in a shorter amount of time,” potentially paving the way for more efficient regeneration of bone. Merzaban co-led the study together with sensor scientist Jürgen Kosel and colleagues from their labs.

The scientists analyzed the bone-producing capability of their nanowire scaffold, both with and without magnetic signals. They patterned the tiny wires in an evenly spaced grid and then layered bone marrow-derived human mesenchymal stem cells (MSCs) on top. Each of the tiny wires is about the size of the tail-like appendage found on some bacteria.

The researchers discovered that adding a low-frequency magnetic field greatly accelerated the process of bone development. Within two days of incubation under mechanical stimulation, genetic markers of bone development could be detected, while genes linked to stemness and self-renewal quickly became inactive. The scientists could also witness the cells rebuilding themselves to become more bone-like at a rapid rate under a microscope.

Next, the KAUST team plans to test its system in mouse models of degenerative bone disease, with the expectation that stem cell–seeded nanowire scaffolds can be safely implanted at sites of injury and promote tissue repair. An externally applied magnetic field would be used to speed the healing process.

Study author Jose Efrain Perez, a former Ph.D. student in Kosel’s lab, also sees potential applications in other disease settings. As he points out: “Varying the matrix stiffness by increasing or decreasing nanowire length and diameter could promote differential responses with MSCs.” Or they could use other types of stem cells to, for example, promote neuronal growth and brain repair after a stroke.

What’s more, Perez adds, “We could further customize the nanowire scaffold itself or the base material — for instance, by using different metals to exploit their magnetic responses or coating the nanowires with biomolecules for potential delivery upon cellular contact.”

For such a small technology, the possibilities are huge.

Reference: “Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells” by Jose E. Perez, Bashaer Bajaber, Nouf Alsharif, Aldo I. Martínez-Banderas, Niketan Patel, Ainur Sharip, Enzo Di Fabrizio, Jasmeen Merzaban and Jürgen Kosel, 16 June 2022, Journal of Nanobiotechnology.
DOI: 10.1186/s12951-022-01488-5

The U.S. made a breakthrough battery discovery — then gave the technology to China


This is the story of the former UniEnergy Technologies in Mukilteo, Wash. where Taxpayers spent $15 million on research to build a breakthrough battery. Then the U.S. government gave it to China -,yes then gave it away to China.

When a group of engineers and researchers gathered in a warehouse in Mukilteo, Wash., 10 years ago, they knew they were onto something big. They scrounged up tables and chairs, cleared out space in the parking lot for experiments and got to work.

They were building a battery — a vanadium redox flow battery — based on a design created by two dozen U.S. scientists at a government lab. The batteries were about the size of a refrigerator, held enough energy to power a house, and could be used for decades. The engineers pictured people plunking them down next to their air conditioners, attaching solar panels to them, and everyone living happily ever after off the grid.

“It was beyond promise,” said Chris Howard, one of the engineers who worked there for a U.S. company called UniEnergy. “We were seeing it functioning as designed, as expected.”

It all began in the basement of a government lab, three hours southwest of Seattle, called Pacific Northwest National Laboratory. It was 2006, and more than two dozen scientists began to suspect that a special mix of acid and electrolyte could hold unusual amounts of energy without degrading. They turned out to be right.

It took six years and more than 15 million taxpayer dollars for the scientists to uncover what they believed was the perfect vanadium battery recipe. Others had made similar batteries with vanadium, but this mix was twice as powerful and did not appear to degrade the way cellphone batteries or even car batteries do. The researchers found the batteries capable of charging and recharging for as long as 30 years.

An employee looks at a vanadium flow battery in Pacific Northwest National Laboratory’s Battery Reliability Laboratory in 2021. Andrea Starr/Pacific Northwest National Laboratory

Gary Yang, the lead scientist on the project, said he was excited to see if he could make the batteries outside the lab. The lab encourages scientists to do just that, in an effort to bring critical new technology into the marketplace. The lab and the U.S. government still hold the patents, because U.S. taxpayers paid for the research.

In 2012, Yang applied to the Department of Energy for a license to manufacture and sell the batteries.

The agency issued the license, and Yang launched UniEnergy Technologies. He hired engineers and researchers. But he soon ran into trouble. He said he couldn’t persuade any U.S. investors to come aboard.

“I talked to almost all major investment banks; none of them (wanted to) invest in batteries,” Yang said in an interview, adding that the banks wanted a return on their investments faster than the batteries would turn a profit.

Imre Gyuk (left), director of energy storage research in the Office of Electricity of the Department of Energy, Washington Gov. Jay Inslee and Gary Yang of UniEnergy Technologies stand together in 2015. Office of Gov. Jay Inslee

He said a fellow scientist connected him with a Chinese businessman named Yanhui Liu and a company called Dalian Rongke Power Co. Ltd., along with its parent company, and he jumped at the chance to have them invest and even help manufacture the batteries.

At first, UniEnergy Technologies did the bulk of the battery assembly in the warehouse. But over the course of the next few years, more and more of the manufacturing and assembling began to shift to Rongke Power, Chris Howard said. In 2017, Yang formalized the relationship and granted Dalian Rongke Power Co. Ltd. an official sublicense, allowing the company to make the batteries in China.

Any company can choose to manufacture in China. But in this case, the rules are pretty clear. Yang’s original license requires him to sell a certain number of batteries in the U.S., and it says those batteries must be “substantially manufactured” here.

In an interview, Yang acknowledged that he did not do that. UniEnergy Technologies sold a few batteries in the U.S., but not enough to meet its requirements. The ones it did sell, including in one instance to the U.S. Navy, were made in China. But Yang said in all those years, neither the lab nor the department questioned him or raised any issues.

Chris Howard is now the director of operations at Forever Energy in Bellevue, Wash. Jovelle Tamayo for NPR

Then in 2019, Howard said, UniEnergy Technologies officials gathered all the engineers in a meeting room. He said supervisors told them they would have to work in China at Rongke Power Co. for four months at a time.

“It was unclear, certainly to myself and other engineers, what the plan was,” said Howard, who now works for Forever Energy.

Solar projects are on hold as U.S. investigates whether China is skirting trade rules

Yang acknowledges that he wanted his U.S. engineers to work in China. But he says it was because he thought Rongke Power could help teach them critical skills.

Yang was born in China but is a U.S. citizen and got his Ph.D. at the University of Connecticut. He said he wanted to manufacture the entire battery in the U.S., but that the U.S. does not have the supply chain he required. He said China is more advanced when it comes to manufacturing and engineering utility-scale batteries.

“In this field — manufacturing, engineering — China is ahead of the U.S.,” Yang said. “Many wouldn’t believe [it].”

He said he didn’t send the battery and his engineers abroad to help China. He said the engineers in that country were helping his UniEnergy Technologies employees and helping him get his batteries built.

But news reports at the time show the moves were helping China. The Chinese government launched several large demonstration projects and announced millions of dollars in funding for large-scale vanadium batteries.

As battery work took off in China, Yang was facing more financial trouble in the U.S. So he made a decision that would again keep the technology from staying in the U.S.

The EU has strict rules about where companies manufacture products

In 2021, Yang transferred the battery license to a European company based in the Netherlands. The company, Vanadis Power, told NPR it initially planned to continue making the batteries in China and then would set up a factory in Germany, eventually hoping to manufacture in the U.S., said Roelof Platenkamp, the company’s founding partner.

Vanadis Power needed to manufacture batteries in Europe because the European Union has strict rules about where companies manufacture products, Platenkamp said.

“I have to be a European company, certainly a non-Chinese company, in Europe,” Platenkamp said in an interview with NPR.

Gary Yang launched UniEnergy Technologies after the Department of Energy issued him a license to manufacture and sell the vanadium batteries. Jovelle Tamayo for NPR

But the U.S. has these types of rules, too. Any transfer of a U.S. government license requires U.S. government approval so that manufacturing doesn’t move overseas. The U.S. has lost significant jobs in recent years in areas where it first forged ahead, such as solar panels, drones and telecom equipment. Still, when UniEnergy requested approval, it apparently had no trouble getting it.

On July 7, 2021, a top official at UniEnergy Technologies emailed a government manager at the lab where the battery was created. The UniEnergy official said they were making a deal with Vanadis, according to emails reviewed by NPR, and were going to transfer the license to Vanadis.

“We’re working to finalize a deal with Vanadis Power and believe they have the right blend of technical expertise,” the email from UniEnergy Technologies said. “Our transaction with Vanadis is ready to go pending your approval …”

The government manager responded that he needed confirmation before transferring the license and emailed a second employee at UniEnergy. The second employee responded an hour and a half later, and the license was transferred to Vanadis Power.

Whether the manager or anyone else at the lab or Department of Energy thought to check during that hour and a half or thereafter whether Vanadis Power was an American company, or whether it intended to manufacture in the U.S., is unclear. Vanadis’ own website said it planned to make the batteries in China.

In response, department officials said they review each transfer for compliance and said that new rules put in place last summer by the Biden administration will close loopholes and keep more manufacturing here.

But agency officials acknowledged that its reviews often rely on “good faith disclosures” by the companies, which means if companies such as UniEnergy Technologies don’t say anything, the U.S. government may never know.

Joanne Skievaski said she and others from the company repeatedly warned Department of Energy officials that the UniEnergy license was not in compliance. Jovelle Tamayo for NPR

That’s a problem that has plagued the department for years, according to government investigators.

In 2018, the Government Accountability Office found that the Department of Energy lacked resources to properly monitor its licenses, relied on antiquated computer systems, and didn’t have consistent policies across its labs.

In this case, it was an American company, Forever Energy, that raised concerns about the license with UniEnergy more than a year ago. Joanne Skievaski said she and others from the company repeatedly warned department officials that the UniEnergy license was not in compliance. In emails NPR has reviewed, department officials told them it was.

“How is it that the national lab did not require U.S. manufacturing?” Skievaski asked. “Not only is it a violation of the license, it’s a violation to our country.”

Now that the Department of Energy has revoked the license, Skievaski said she hopes Forever Energy will be able to acquire it or obtain a similar license. The company plans to open a factory in Louisiana next year and begin manufacturing. She bristles at the idea that U.S. engineers aren’t up to the challenge.

“That’s hogwash,” she said. “We are ready to go with this technology.”

Still, she says it will be difficult for any American company at this point to catch up. Industry trade reports currently list Dalian Rongke Power Co. Ltd. as the top manufacturer of vanadium redox flow batteries worldwide. Skievaski also worries about whether China will stop making the batteries once an American company is granted the right to start making them.

That may be unlikely. Chinese news reports say the country is about to bring online one of the largest battery farms the world has ever seen. The reports say the entire farm is made up of vanadium redux flow batteries.

This story is a partnership with NPR’s Station Investigations Team, which supports local investigative journalism, and the Northwest News Network, a collaboration of public radio stations that broadcast in Oregon and Washington state.